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KMID : 0376619950200020073
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Seoul Journal of Psychiatry
1995 Volume.20 No. 2 p.73 ~ p.79
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Developmental Changes of MAPK Activation in Rat Brain after Electroconvulsive Shock
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김덕환
전송희/강웅구/주연호/조수철/김용식/박주배
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Abstract
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Signal transduction via Mitogen Activated Protein Kinases(MAPKs) is supposed to play an important role in the regulation of neuronal function. In order to elucidate the mechanism of activation of MAPKs in rat brain, we have observed tyrosine
phosphorylation of MAPKs after electroconvulsive shock(ECS) in developing rat brain. Rats of various age 7(P7), 14(P14) and 21(P21) days postnatal) were given ECS and their hippocampi and cerebral corteces were dissected out at appropriate time
points.
The amount of MAPK proteins and MAPK tyrosine phosphorylation were determined by immunoblot analysis. P7 rats showed considerable amount of MAPKs but no tyrosine phosphorylation was detected in both hippocampus and cerebral cortex. P14 rats
showed
transient increase in tyrosine phosphorylation of 42kDa MAPK only in hippocampus, while P21 rats showed the increase in both tissues. The phosphorylation reached peak level at 2 minutes and disappeard within 10 to 15 minutes after ECS, and these
pattern
is compatible with those of adult rats. These results suggest that the MAPK cascade undergoes maturational process during early stage of brain development.
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KEYWORD
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